Rising star Asselstine weighed in at 128lbs, with former world title challenger Oliver Lontchi weighing in at 124lbs.
Asselstine islooking to bounce straight back after suffering a questionable split decision loss against Baha Laham in his last outing.
The 26-year-old Toronto featherweight boasts 12 wins with seven knockouts and only the single defeat on his record.
But, he cannot afford to underestimate his opponent in front of his home crowd.
Montreal man Lontchi, 29, is a former world title challenger and with 18 wins, eight by knockout, two defeats with two knockouts and two draws to his name, will certainly be no pushover.
Also on the bill, Samuel Vargas weighed in at 153lbs as he prepares to take on on Julius Bunda (who also weighed in at 153lbs) over 10 rounds for the NABA-Canada super welterweight title.
Vargas, known as Canada’s most ferocious fighter, has 10 wins with one draw and no defeats to his name and will be looking to make it win number 11.
But, he meets one of the hardest punchers in the division with Bunda, who, despite a record of just five wins with two defeats and two draws, will certainly be a stern test.
The fights take place at the 28th Annual Shaw Evening of Boxing in support of the Shaw Festival of the Arts, Fairmont Royal York, Toronto on April 9.
The evening will also feature George Chuvalo as a special guest.
Tyler Asselstine 128lbs vs Oliver Lontchi 124lbs
Samual Vargas 153lbs vs Juluis Bunda 153lbs